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KEYENCE
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Carl Zeiss
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Image Search Results
Journal: Journal of neurovirology
Article Title: Transgenic Mice Expressing HIV-1 Envelope Protein gp120 in the Brain as an Animal Model in NeuroAIDS Research
doi: 10.1007/s13365-017-0584-2
Figure Lengend Snippet: Sagittal brain sections of 6 months-old gp120-transgenic and wild-type (WT) littermate controls were immune-stained for neuronal MAP-2 (red) and astrocytic GFAP (green). DNA (blue) was labeled with H33342 and is shown to indicate nuclei. Fluorescence-labeled sections were analyzed using a Zeiss Axiovert 100 M inverted microscope and Slidebook software (Intelligent Imaging Innovations, Denver, CO) to record Z-stacks and perform deconvolution and 3D reconstruction. The upper six panels show 3D volume views, the bottom two panel are 3D surface views. Representative areas of mid-frontal cortex, layer 3, are shown. Note the difference in the density of MAP-2 immunoreactive neuropil and astrocyte morphology between WT and gp120tg samples, and the dimensions of the 10 μm grid.
Article Snippet: Fluorescence-labeled sections were analyzed using a Zeiss Axiovert 100 M inverted microscope and
Techniques: Transgenic Assay, Staining, Labeling, Fluorescence, Inverted Microscopy, Software, Imaging